Samus is the only character representation of the Metroid universe. Samus' sprites were ripped from Metroid Fusion. Curiously, those sprites were not from Samus, but were taken from her clone-enemy, the SA-X. Samus uses a variety of beams in her attacks. Mega Man X uses a similar moveset so he could be considered a semi-clone of her.
Samus is ranked 14th on the tier list. Samus has the longest non-projectile range in the game with her machine gun attacks. These attacks also double as Samus' instant kill moves, giving her some of the best kill options in the game. She has good close range options, as her Morph Ball has the smallest hitbox out of all the rolling moves and her Screw Attack allows her to deal damage when jumping.
However, Samus' machine gun attacks erroneously increase her hurtboxes to ridiculous sizes, allowing enemies to hurt her by attacking the beam instead. All three of these attacks also have a long cooldown time shared between them, preventing her from using half of her moveset until it expires. In addition, her Power Beam attack is overall lackluster and has a slow firing rate. Her mobility is only average at best as she can be difficult to control when turning since her sprite is not centered properly.
Samus' pros slightly overshadow her cons, allowing her to rank in the higher mid tier.
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Additionally, her jump animation is the Screw Attack, who also serves as an attack.
Samus is one of the only two playable characters in the original Super Smash Flash Demo, the other being Blade. It's likely that she was programmed over other unknown data left over from when SSF was going to be a platform game, as she's scaled down to match Blade's height and is referred to in the debug indicators as "Enemy". She also had a significantly faster rate of fire in the demo, which was toned down later on to be less overpowered.
Samus's sprites are not of Samus at all, but of the SA-X, a clone of her that appears in Metroid Fusion which was born from removed pieces of her armor infected by the lethal, intelligent X Parasite.